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Development and characterization of monoclonal antibody to the immunoglobulin of turbot (Scophthalmus maximus)

Wang Weifang,Li Qingmei,Chai Shujun,Gan Lingling,Hong Lei,Guo Junqing,Lei Jilin

Strategic Study of CAE 2014, Volume 16, Issue 9,   Pages 16-20

Abstract: All the mAbs (denominated B1D1, D5C2, E1B2 and F4A1) are showed good anti-turbot IgM reactivity in enzyme

Keywords： turbot (Scophthalmus     maximus)     immunoglobulin M (IgM)     monoclonal antibody     BALB/C mice    

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immune mapped protein 1 (NcIMP1) is a novel vaccine candidate against neosporosis

Xia CUI,Daoyu YANG,Tao LEI,Hui WANG,Pan HAO,Qun LIU

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 1,   Pages 66-72 doi: 10.15302/J-FASE-2015047

Abstract: resulting in the recombination plasmid pcDNA-IMP1, which was used for the intramuscular immunization of BALB/c mice.Quantification of the cerebral parasite burden of mice challenged with 2 × 10 was performed 14 daysThe results showed that the mice immunized with pcDNA-IMP1 developed a high level of specific antibodyThe parasite burden was significantly reduced in the pcDNA-IMP1-vaccinated mice.

Keywords： Neospora caninum     immune mapped protein 1 (IMP1)     vaccine candidate     BALB/c mice    

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Carbon dots-based fluorescence sensor for two-photon imaging of pH in diabetic mice

Frontiers of Chemical Science and Engineering 2023, Volume 17, Issue 3,   Pages 298-306 doi: 10.1007/s11705-022-2212-9

Abstract: CA-CDs has been successfully applied for two-photon imaging of the pH in liver and kidney of diabetic miceImaging results showed that the pH value in kidney of diabetic mice was lower than that of the normalmice, while the pH value in liver of diabetic mice was almost the same as that of the normal mice.

Keywords： carbon dots     two-photon imaging     pH     diabetic mice    

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Inhibitory activity of Bifidobacterium adolescent combined with cisplatin on melanoma in mice and its

HUANG Hongying, LIU Guangchao, QI Yijun, DU Yaowu, CHEN Jugao, MA Yuanfang

Frontiers of Medicine 2008, Volume 2, Issue 2,   Pages 186-190 doi: 10.1007/s11684-008-0035-9

Abstract: inhibitory activity of Bifidobacterium adolescent combined with cisplatin on the growth of melanoma (B16) in miceC57 mice were inoculated with B16 cancer cells to construct mouse model of melanoma and treated withThe ratio of inhibitory activity reached 74.45% when the mice were treated by bifidobacterium combinedthe inhibitory activity on B16 cell proliferation is correlated with the enhanced immune activity in mice
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Particulate matter 2.5 triggers airway inflammation and bronchial hyperresponsiveness in mice by activating

Frontiers of Medicine 2021, Volume 15, Issue 5,   Pages 750-766 doi: 10.1007/s11684-021-0839-4

Abstract: acetylation, airway inflammation, and bronchial hyperresponsiveness were deteriorated in SIRT2 knockout mice

Keywords： particulate matter 2.5     sirtuin 2     p65     airway inflammation     bronchial hyperresponsiveness     triptolide    

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Immunogenicity and protective immunity against otitis media caused by pneumococcus in mice of Hib conjugate

Zeyu Chen,Rong Guo,Jianghong Xu,Chuangjun Qiu

Frontiers of Medicine 2016, Volume 10, Issue 4,   Pages 490-498 doi: 10.1007/s11684-016-0470-y

Abstract: polysaccharide conjugate vaccine with the pneumococcal surface adhesin A (PsaA) protein carrier in young miceA total of 15 young mice aged 3 weeks to 5 weeks were immunized with the conjugate vaccine, and another15 young mice of the same age were immunized with the licensed Hib-tetanus toxoid (TT) vaccine.Furthermore, the third group of 15 young mice was inoculated with phosphate buffer saline as control.The immunized mice were inoculated with pneumococcus in the middle ear.

Keywords： conjugate vaccine     pneumococcal surface adhesin A     Hemophilus influenzae b     immunogenicity     otitis media    

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Gene therapy for hemophilia B mice with scAAV8-LP1-hFIX

Wei Lu,Qingzhang Zhou,Hao Yang,Hao Wang,Yexing Gu,Qi Shen,Jinglun Xue,Xiaoyan Dong,Jinzhong Chen

Frontiers of Medicine 2016, Volume 10, Issue 2,   Pages 212-218 doi: 10.1007/s11684-016-0438-y

Abstract:

Hemophilia B is a hemorrhagic disease caused by the deficiency of clotting factor IX (FIX). Gene therapy might be the ultimate strategy for the disease. However, two main problems that should be solved in gene therapy for hemophilia B are immunity and safety. Self-complementary adeno-associated virus serotype 8 (scAAV8), a non-human primate AAV featuring low immunogenicity and high transfection efficiency in liver cells, might be a potential vector for hemophilia B gene therapy. A strong liver-specific promoter-1 (LP1) was inserted and mutant human FIX Arg338Ala was introduced into plasmid scAAV8-LP1 to develop an optimized AAV8 vector that expresses human clotting factor FIX (hFIX). The efficiency of scAAV8-LP1-hFIX administered through normal systemic injection or hydrodynamic injection was compared. A high expression was achieved using hydrodynamic injection, and the peak hFIX expression levels in the 5×1011 and 1×1011 virus genome (vg) cohorts were 31.94% and 25.02% of normal level, respectively, at 60 days post-injection. From the perspective of long-term (200 days) expression, both injection methods presented promising results with the concentration value maintained above 4% of normal plasma. The results were further verified by enzyme-linked immunosorbent assay and activated partial thromboplastin time. Our study provides a potential gene therapy method for hemophilia B.

Keywords： hemophilia B     AAV8     hFIX     gene therapy    

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Overexpression of netrin-1 improves neurological outcomes in mice following transient middle cerebral

Haiyan LU, Yongting WANG, Falei YUAN, Jianrong LIU, Lili ZENG, Guo-Yuan YANG

Frontiers of Medicine 2011, Volume 5, Issue 1,   Pages 86-93 doi: 10.1007/s11684-011-0118-x

Abstract: The level of NT-1 was increased in the AAV-NT-1-transduced tMCAO mice compared with the control mice.Furthermore, the neurobehavioral outcomes were significantly improved in AAV-NT-1-transduced mice compared

Keywords： adeno-associated virus     angiogenesis     gene transfer     ischemia     middle cerebral artery occlusion     netrin-1    

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Long-term correction of hemorrhagic diathesis in hemophilia A mice by an AAV-delivered hybrid FVIII composed

Frontiers of Medicine 2022, Volume 16, Issue 4,   Pages 584-595 doi: 10.1007/s11684-021-0844-7

Abstract: (hLC), and rat light chain (rLC) were transfected into cells and hydrodynamically injected into HA micePurified AAV viruses were intravenously injected into HA mice at two doses.Notably, the HA mice undergoing the AAV-delivered hHC+ rLC treatment exhibited a visibly higher activity+ rLC) via an AAV-delivered gene therapy substantially improved the hemorrhagic diathesis of the HA mice

Keywords： hemophilia A     adeno-associated virus (AAV)     human/rat hybrid factor VIII     gene therapy     dual chain strategy    

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Gut Microbiota-Controlled Tryptophan Metabolism Improves D-Gal/LPS-Induced Acute Liver Failure in C57BL/6 Mice Article

Zhipeng Zheng, Li Wu, Yuqiu Han, Jun Chen, Shuai Zhu, Yuanyuan Yao, Baohong Wang, Lanjuan Li

Engineering 2022, Volume 14, Issue 7,   Pages 134-146 doi: 10.1016/j.eng.2020.12.013

Abstract: improved the survival of D-(+)-galactosamine hydrochloride (D-Gal)/lipopolysaccharide (LPS)-induced ALF in C57BL/6 mice.inflammatory responses were inhibited and metabolic pathways were upregulated in the liver of Abx-treated ALF miceFurthermore, AhR-targeted treatments affected the outcome of ALF mice with or without Abx pretreatment

Keywords： Gut microbiota     Antibiotic     Tryptophan     Kynurenine     Aryl hydrocarbon receptor     Acute liver failure    

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Prediction of Driver Gene Matching in Lung Cancer NOG/PDX Models Based on Artificial Intelligence Article

Yayi He, Haoyue Guo, Li Diao, Yu Chen, Junjie Zhu, Hiran C.

Engineering 2022, Volume 15, Issue 8,   Pages 102-114 doi: 10.1016/j.eng.2021.06.017

Abstract:

Patient-derived tumor xenografts (PDXs) are a powerful tool for drug discovery and screening in cancer. However, current studies have led to little understanding of genotype mismatches in PDXs, leading to massive economic losses. Here, we established PDX models from 53 lung cancer patients with a genotype matching rate of 79.2% (42/53). Furthermore, 17 clinicopathological features were examined and input in stepwise logistic regression (LR) models based on the lowest Akaike information criterion (AIC), least absolute shrinkage and selection operator (LASSO)-LR, support vector machine (SVM) recursive feature elimination (SVM-RFE), extreme gradient boosting (XGBoost), gradient boosting and categorical features (CatBoost), and the synthetic minority oversampling technique (SMOTE). Finally, the performance of all models was evaluated by the accuracy, area under the receiver operating characteristic curve (AUC), and F1 score in 100 testing groups. Two multivariable LR models revealed that age, number of driver gene mutations, epidermal growth factor receptor (EGFR) gene mutations, type of prior chemotherapy, prior tyrosine kinase inhibitor (TKI) therapy, and the source of the sample were powerful predictors. Moreover, CatBoost (mean accuracy = 0.960; mean AUC = 0.939; mean F1 score = 0.908) and the eight-feature SVM (mean accuracy = 0.950; mean AUC = 0.934; mean F1 score = 0.903) showed the best performance among the algorithms. Meanwhile, application of the SMOTE improved the predictive capability of most models, except CatBoost. Based on the SMOTE, the ensemble classifier of single models achieved the highest accuracy (mean = 0.975), AUC (mean = 0.949), and F1 score (mean = 0.938). In conclusion, we established an optimal predictive model to screen lung cancer patients for NOD/Shi-scid, interleukin-2 receptor (IL-2R) γnull (NOG)/PDX models and offer a general approach for building predictive models.

Keywords： Machine learning     Patient-derived tumor xenografts     NOG mice    

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Development of the expressed immunoglobulin μ chain repertoire during maturation of mice B cells

Jingwen LIANG,Yingfeng LUO,Yi SUN,Meng LEI,Bing ZHANG,Songnian HU,Yaofeng ZHAO

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3,   Pages 201-213 doi: 10.15302/J-FASE-2014017

Abstract: In the bone marrow and spleen, the developing B cell populations undergo both negative and positive selections to shape their B cell receptor repertoire. To gain insight into the shift of the immunoglobulin heavy (IgH) chain repertoire during B cell development, we undertook large scale Ig μ chain repertoire analysis of pre-B, immature B and spleen B cell populations. We found that the majority of V gene segments, V families, J and D gene segments, were observed to have significantly different usage frequencies when three B cell populations were compared, but the usage profile of the V D, and J genes between different B cell populations showed high correlations. In both productive and nonproductive rearrangements, the length of CDRH3 shortened significantly on average when B cells entered the periphery. However, the CDRH3 length distribution of nonproductive rearrangements did not follow a Gaussian distribution, but decreased successively in the order 3 -2, 3 -1 and 3 , suggesting a direct correlation between mRNA stability and CDRH3 length patterns of nonproductive rearrangements. Further analysis of the individual components comprising CDRH3 of productive rearrangements indicated that the decrease in CDRH3 length was largely due to the reduction of N addition at the 5′ and 3′ junctions. Moreover, with development, the amino acid content of CDRH3 progressed toward fewer positively charged and nonpolar residues but more polar residues. All these data indicated that the expressed Ig μ chain repertoire, especially the repertoire of CDRH3, was fine-tuned when B cells passed through several checkpoints of selection during the process of maturation.

Keywords： repertoire     complementarity determining region 3 of the IgH chain (CDRH3)     immunoglobulin     heavy chain     variable region    

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Valveless pumping using a two-stage impedance pump

C.-C. LEE, Y. A. ABAKR, K.-C. WOO

Frontiers of Mechanical Engineering 2013, Volume 8, Issue 3,   Pages 311-318 doi: 10.1007/s11465-013-0270-x

Abstract:

Impedance pump is defined as a type of valveless pumping mechanism, where an elastic tube is joined with a tube of different impedance, a periodic asymmetrical compression on part of the elastic tube will produce a unidirectional flow. Numerical and experimental studies had been conducted in the past, but little experimental work was found to exploit the principle of multi-stage impedance pumping for fluid transportation. This paper concentrates on the fluid behavior for an open-loop two-stage system experimentally. The aim of this work is to have an insight on the deliverable head and pumping rate of an open-loop two-stage impedance pumping system. This paper generally investigates the effect of different parameters towards the generation of unidirectional flows and composite flow of two opposite directions. Results obtained are of significant values.

Keywords： valveless pumping     open-loop     two-stage     unidirectional     composite    

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Dynein axonemal heavy chain 10 deficiency causes primary ciliary dyskinesia in humans and mice

Frontiers of Medicine   Pages 957-971 doi: 10.1007/s11684-023-0988-8

Abstract: Using exome sequencing, we identified a novel DNAH10 homozygous variant (c.589C > T, p.R197W)Subsequently, animal models of Dnah10-knockin mice harboring missense variants and Dnah10-knockout mice recapitulated the phenotypes of PCD, including chronic respiratory infection, male infertility

Keywords： DNAH10     mice     motile cilia     mutation     primary ciliary dyskinesia    

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Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 3,   Pages 242-248 doi: 10.15302/J-FASE-2015059

Abstract: further study the functions and mechanisms of lactoferrin, we modified the lactoferrin promoter of miceSeven mice with lactoferrin promoter mutations were obtained with an efficiency of 24% (7/29) by injectingplasmid pX330, expressing a small guide RNA and human codon-optimized SpCas9, into fertilized eggs of micepronuclear injection of a circular plasmid is a feasible and efficient method for targeted mutagenesis in mice

Keywords： lactoferrin     promoter     CRISPR/Cas9     plasmid pX330    

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Title Author Date Type Operation

Development and characterization of monoclonal antibody to the immunoglobulin of turbot (Scophthalmus maximus)

Wang Weifang,Li Qingmei,Chai Shujun,Gan Lingling,Hong Lei,Guo Junqing,Lei Jilin

Journal Article

immune mapped protein 1 (NcIMP1) is a novel vaccine candidate against neosporosis

Xia CUI,Daoyu YANG,Tao LEI,Hui WANG,Pan HAO,Qun LIU

Journal Article

Carbon dots-based fluorescence sensor for two-photon imaging of pH in diabetic mice

Journal Article

Inhibitory activity of Bifidobacterium adolescent combined with cisplatin on melanoma in mice and its

HUANG Hongying, LIU Guangchao, QI Yijun, DU Yaowu, CHEN Jugao, MA Yuanfang

Journal Article

Particulate matter 2.5 triggers airway inflammation and bronchial hyperresponsiveness in mice by activating

Journal Article

Immunogenicity and protective immunity against otitis media caused by pneumococcus in mice of Hib conjugate

Zeyu Chen,Rong Guo,Jianghong Xu,Chuangjun Qiu

Journal Article

Gene therapy for hemophilia B mice with scAAV8-LP1-hFIX

Wei Lu,Qingzhang Zhou,Hao Yang,Hao Wang,Yexing Gu,Qi Shen,Jinglun Xue,Xiaoyan Dong,Jinzhong Chen

Journal Article

Overexpression of netrin-1 improves neurological outcomes in mice following transient middle cerebral

Haiyan LU, Yongting WANG, Falei YUAN, Jianrong LIU, Lili ZENG, Guo-Yuan YANG

Journal Article

Long-term correction of hemorrhagic diathesis in hemophilia A mice by an AAV-delivered hybrid FVIII composed

Journal Article

Gut Microbiota-Controlled Tryptophan Metabolism Improves D-Gal/LPS-Induced Acute Liver Failure in C57BL/6 Mice

Zhipeng Zheng, Li Wu, Yuqiu Han, Jun Chen, Shuai Zhu, Yuanyuan Yao, Baohong Wang, Lanjuan Li

Journal Article

Prediction of Driver Gene Matching in Lung Cancer NOG/PDX Models Based on Artificial Intelligence

Yayi He, Haoyue Guo, Li Diao, Yu Chen, Junjie Zhu, Hiran C.

Journal Article

Development of the expressed immunoglobulin μ chain repertoire during maturation of mice B cells

Jingwen LIANG,Yingfeng LUO,Yi SUN,Meng LEI,Bing ZHANG,Songnian HU,Yaofeng ZHAO

Journal Article

Valveless pumping using a two-stage impedance pump

C.-C. LEE, Y. A. ABAKR, K.-C. WOO

Journal Article

Dynein axonemal heavy chain 10 deficiency causes primary ciliary dyskinesia in humans and mice

Journal Article

Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG

Journal Article